A promising biotechnical approach to pest management of the western corn rootworm in Illinois maize fields shielded with a MCA kairomone baited trap line

The leaf beetle Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae), (D.v.v.), also called the western corn rootworm, is endemic to the New World and ranks among the top ten insect pests in worldwide grain production. D.v.v. causes annual damages of 1 billion US Dollars and is a notoriously difficult insect pest to control and manage, as entomological history of the past 50 years amply demonstrates (METCALF 1986). Considering recent emphasis on environmentally compatible and sustainable management strategies, entomologists and practitioners are encouraged to pay increased attention to novel approaches such as biotechnial methods which today are characterized by preferential use of signal compounds. Fortunately, both insect and plants provide a wide variety of such natural resources. In the case of D.v.v., sex pheromonesand plant kairomones as specific attractants and management tools are relatively well investigated through numerous contributions by GUSS et al. (1982), METCALF & METCALF (1992), METCALF (1994) and many recent publications on the advance and spread of D.v.v. within Europe (BERGER 1995-2004, HUMMEL 2003). Principle of MSD method: In this paper, the plant kairomone 4-methoxycinnamaldehyde (MCA), a specific attractant for D.v.v., is being used as a tool within the newly proposed "MSD" strategy. It combines a two pronge approach consisting as the well known mass trapping with the novel shielding and deflecting, called in short "diversion" and introduced here for the first time. An invisible “curtain” or “fence” of MCA vapor released from a MCA trap line establishes a behavioral barrier which the flying beetles cannot easily pass without being 1. either caught in one of the high capacity traps or 2. being diverted elsewhere. The net effect is a significant reduction in adult population density and oviposition within the MCA treated field as compared to an untreated control field. These effects can be experimentally measured by 1. adult beetle counts on maize plants, 2. by counts in independent monitoring traps baited with the D.v.v. sex pheromone, and 3. by egg counts taken in soil samples.Umweltgerechter und nachhaltiger Pflanzenschutz erfordert eine Vielzahl verschiedener Strategien zum Management von Schadinsekten auf ihren Wirtspflanzen. Insekten- und pflanzeneigene Signalstoffe sowie ihre synthetischen Analoga bieten einen nahezu unerschöpflichen Vorrat spezifischer Lockwirkungen und Interventionsmöglichkeiten an, der bisher leider nur unzureichend genutzt wird. Kürzliche Entdeckungen sowohl neuer Kairomon-Lockstoffe als auch neuer Verfahrensschritte für das Management des Westlichen Maiswurzelbohrers Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) (D.v.v.) erweitern das Spektrum anwendbarer Management- Optionen für diesen schwer bekämpfbaren Schädling im nordamerikanischen Maisanbau. Seit seiner Einschleppung nach Europa (ČAMPRAG & BAČA 1995) wird D.v.v. neuerdings auch zunehmend zum Problemschädling an Mais in Europa. Die neue "MSD"-Technik besteht aus einer Kombination von Massenabfang ("mass trapping"), Abschirmung ("shielding") und Umlenkung ("diversion") der Blattkäfer. Fallen hoher Fangkapazität, die das Kairomon 4-Methoxyzimtaldehyd (MCA) und Cucurbitacin- Pulver als fraßfördernden Stoff enthalten, können bei Aufstellung als "Fallenzaun" relativ geringer Dichte die Käfer so umlenken, dass zwischen den beiden Seiten dieses Zauns eine unsichtbare Geruchs-Barriere entsteht. Diese führt zu einer messbaren und signifikanten Verminderung der Käferzahl zwischen MCA behandelten Feldabschnitten gegenüber ihren unbehandelten Kontrollen. In 0,27 ha bzw. 0,12 ha großen Maisversuchsfeldern der Standorte Urbana und Champaign des US-Staates Illinois ließen sich während der Monate August und September 2003 und 2004 nach MCA-Behandlung gegenüber Kontrollen deutliche und signifikante Verminderungen von D.v.v. an Hand dreier Kriterien nachweisen: 1. Käferzahlen auf Maispflanzen innerhalb des "MSD-Feldes", 2. Käferzahlen in Sexuallockstoff-Fallen im MSD Feld und 3. die Zahl abgelegter D.v.v.-Eier im Boden des MSD Feldes nehmen ab. Der beobachtete Effekt läßt sich nicht allein auf die Populationsverminderung infolge hoher Abfangzahlen zurückführen. Es gibt darüber hinaus einen Abschirm- und Umlenkeffekt, dessen sinnes- und verhaltensphysiologische Mechanismen zusätzliche künftige Erforschungen erforderlich machen

Massive envelopes and filaments in the NGC 3603 star forming region

The formation of massive stars and their arrival on the zero-age main-sequence occurs hidden behind dense clouds of gas and dust. In the giant Hii region NGC 3603, the radiation of a young cluster of OB stars has dispersed dust and gas in its vicinity. At a projected distance of 2:5 pc from the cluster, a bright mid-infrared (mid-IR) source (IRS 9A) had been identified as a massive young stellar object (MYSO), located on the side of a molecular clump (MM2) of gas facing the cluster. We investigated the physical conditions in MM2, based on APEX sub-mm observations using the SABOCA and SHFI instruments, and archival ATCA 3 mm continuum and CS spectral line data. We resolved MM2 into several compact cores, one of them closely associated with IRS 9A. These are likely infrared dark clouds as they do not show the typical hot-core emission lines and are mostly opaque against the mid-IR background. The compact cores have masses of up to several hundred times the solar mass and gas temperatures of about 50 K, without evidence of internal ionizing sources. We speculate that IRS 9A is younger than the cluster stars, but is in an evolutionary state after that of the compact cores

Genome-wide SNP typing of ancient DNA: Determination of hair and eye color of Bronze Age humans from their skeletal remains.

Objective A genome-wide high-throughput single nucleotide polymorphism (SNP) typing method was tested with respect of the applicability to ancient and degraded DNA. The results were compared to mini-sequencing data achieved through single base extension (SBE) typing. The SNPs chosen for the study allow to determine the hair colors and eye colors of humans. Material and methods The DNA samples were extracted from the skeletal remains of 59 human individuals dating back to the Late Bronze Age. The 3,000 years old bones had been discovered in the Lichtenstein Cave in Lower Saxony, Germany. The simultaneous typing of 24 SNPs for each of the ancient DNA samples was carried out using the 192.24 Dynamic Array (TM) by Fluidigm (R). Results Thirty-eight of the ancient samples (=64%) revealed full and reproducible SNP genotypes allowing hair and eye color phenotyping. In 10 samples (=17%) at least half of the SNPs were unambiguously determined, in 11 samples (=19%) the SNP typing failed. For 23 of the 59 individuals, a comparison of the SNP typing results with genotypes from an earlier performed SBE typing approach was possible. The comparison confirmed the full concordance of the results for 90% of the SNP typings. In the remaining 10% allelic dropouts were identified. Discussion The high genotyping success rate could be achieved by introducing modifications to the preamplification protocol mainly by increasing the DNA input and the amplification cycle number. The occurrence of allelic dropouts indicates that a further increase of DNA input to the preamplification step is desirable

Intranasal sodium citrate solution improves olfaction in post-viral hyposmia

Background: Calcium plays an integral role in olfactory signal transduction, including feedback inhibition. Sodium citrate acts as a calcium sequestrant and when applied intranasally, reduces free calcium available for feedback inhibition, which should theoretically improve olfaction. We aimed to investigate the utility of intranasal sodium citrate in improving the olfactory function of hyposmic patients, by performing this prospective placebo controlled, single-blinded trial. Methodology: Monorhinal olfactory testing for odour identification and threshold was performed in hyposmic patients using “Sniffin’ Sticks”, before and after treatment. Treatment consisted of sodium citrate solution application to the olfactory cleft. Sodium chloride solution was applied to the contralateral olfactory cleft, which therefore acted as placebo control. Patients were blinded to the side of sodium citrate application, and side of treatment was randomized between patients. Results: 57 patients participated in the trial, aged 22-79. Causes of hyposmia included: post-viral (7); posttraumatic (10); sinonasal (30) and idiopathic (10). Compared with placebo, there was significant improvement in the identification scores of participants with post-viral hyposmia, following sodium citrate treatment. No significant change in olfactory function occurred for either identification or threshold in any other aetiological subgroup. Conclusions: Intranasal sodium citrate may be of benefit to patients with post-viral hyposmia

In Healthy Subjects Nasal Nitric Oxide Does Not Correlate with Olfactory Sensitivity, Trigeminal Sensitivity, and Nasal Airflow

Objective: The aim of the study was to determine the relationship between nasal nitric oxide (nNO) and olfactory sensitivity, trigeminal sensitivity and nasal airflow in healthy subjects. Study design: This is a correlational study. Setting: This study was carried out in a tertiary referral centre. Participants: Forty healthy participants were recruited. Main outcome measures: nNO was measured using a chemiluminescence analyser (Niox Vero® , Circassia AB, Uppsala, Sweden), olfactory sensitivity was determined using phenyl ethyl alcohol odour thresholds using the 'Sniffin' Sticks', trigeminal sensitivity was assessed with carbon dioxide delivered by an automated device, and nasal airflow was measured using the peak nasal inspiratory flow (PNIF). Results: The median nNO was 518 ppb (IQR =333) in the right nostril, and it was 567 ppb (IQR = 314) in the left nostril. The median odour threshold was 7.1 (IQR = 4.4), the median CO2 threshold was 919 ms (IQR = 1297) and the mean PNIF was 108 L/min (SEM = 4.9). nNO did not correlate significantly with odour threshold, CO2 threshold or PNIF (Spearman's |ρ| .18). Conclusion: In healthy subjects, nNO does not appear to be associated with olfactory sensitivity, trigeminal sensitivity and PNIF.info:eu-repo/semantics/publishedVersio

Zinc Gluconate in the Treatment of Dysgeusia—a Randomized Clinical Trial

In the treatment of dysgeusia, the use of zinc has been frequently tried, with equivocal results. The aim of the present randomized clinical trial, which involved a sufficiently large sample, was therefore to determine the efficacy of zinc treatment. Fifty patients with idiopathic dysgeusia were carefully selected. Zinc gluconate (140 mg/day; n = 26) or placebo (lactose; n = 24) was randomly assigned to the patients. The patients on zinc improved in terms of gustatory function (p < 0.001) and rated the dysgeusia as being less severe (p < 0.05). Similarly, signs of depression in the zinc group were less severe (Beck Depression Inventory, p < 0.05; mood scale, p < 0.05). With the exception of the salivary calcium level, which was higher in the zinc patients (p < 0.05), no other significant group differences were found. In conclusion, zinc appears to improve general gustatory function and, consequently, general mood scores in dysgeusia patients

Constraints on Cold Dark Matter in the Gamma-ray Halo of NGC 253

A gamma-ray halo in a nearby starburst galaxy NGC 253 was found by the CANGAROO-II Imaging Atmospheric Cherenkov Telescope (IACT). By fitting the energy spectrum with expected curves from Cold Dark Matter (CDM) annihilations, we constrain the CDM-annihilation rate in the halo of NGC 253. Upper limits for the CDM density were obtained in the wide mass range between 0.5 and 50 TeV. Although these limits are higher than the expected values, it is complementary important to the other experimental techniques, especially considering the energy coverage. We also investigate the next astronomical targets to improve these limits.Comment: 13 pages, 5 figures, aastex.cls, natbib.sty, To appear in ApJ v596n1, Oct. 10, 200

Kato's theorem and ultralong-range Rydberg molecules

We consider non-adiabatic coupling in the "trilobite"-like long-range Rydberg molecules created by perturbing degenerate high-$\ell$ Rydberg states with a ground-state atom. Due to the flexibility granted by the high Rydberg level density, the avoided crossings between relevant potential energy curves can become extremely narrow, leading to highly singular non-adiabatic coupling. We find that the gap between the trilobite potential curve and neighboring "butterfly" or "dragonfly" potential curves can even vanish, as in a conical intersection, if the gap closes at an internuclear distance which matches a node of the $s$-wave radial wave function. This is an unanticipated outcome of Kato's theorem

A Search for Active Galactic Nuclei in Sc Galaxies with H II Spectra

(Abridged) We have searched for nuclear radio emission from a statistically complete sample of 40 Sc galaxies within 30 Mpc that are optically classified as star-forming objects, in order to determine whether weak AGNs might be present. Only three nuclear radio sources were detected, in NGC 864, NGC 4123, and NGC 4535. These galaxies have peak 6-cm radio powers of 10^{20} W/Hz at arcsecond resolution, while upper limits of the non-detected galaxies typically range from 10^{18.4} to 10^{20} W/Hz. The three nuclear radio sources all are resolved and appear to have diffuse morphologies, with linear sizes of ~300 pc. This strongly indicates that circumnuclear star formation has been detected in these three H II galaxies. Comparison with previous 20-cm VLA results for the detected galaxies shows that the extended nuclear radio emission has a flat spectrum in two objects, and almost certainly is generated by thermal emission from gas ionized by young stars in the centers of those galaxies. The 6-cm radio powers are comparable to predictions for thermal emission that are based on the nuclear H-alpha luminosities, and imply nuclear star formation rates of 0.08-0.8 solar masses/yr, while the low-resolution NRAO VLA Sky Survey implies galaxy-wide star formation rates of 0.3-1.0 solar masses/yr in stars above 5 solar masses. Although the presence of active nuclei powered by massive black holes cannot be definitively ruled out, the present results suggest that they are likely to be rare in these late-type galaxies with H II spectra.Comment: To appear in ApJ. 7 page